Idpfb 15ml
6/08/Supernatant is transferred to 15ml LoBind tube with extra 4 mL lysis buffer 100 μL of washed and preequilibrated NiNTA resin in lysis buffer was added to the supernatant and rotated overnight, 4°C before washing 1x in lysis buffer, 1x wash buffer (8 M Urea, 01 M Na 2 HPO 4 /NaH 2 PO 4, 10 mM TrisHCl (pH8), 0005 M imidazole, 001 M β−ME), 2x wash buffer 01% Triton X.
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Idpfb 15ml のギャラリー
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