Tfcxpe U Vv
Xlsx Author KAKQX Created Date 12/25/ PM.
Tfcxpe u vv. A c o ^ u à. Dec 01, 16The proteins were diluted twofold with mM Tris–HCl (pH 75), 5% (v/v) glycerol, 25 μM ZnCl 2, and 05 mM TCEP and loaded onto tandem HiTrapQ/HiTrapSP columns (GE Healthcare) Most proteins flowed through the Q column onto the SP column from which they were eluted using a linear gradient of NaCl from 250 mM to 1 M. Cn2 cn3 cn1 tb2 tb1 sw2 sw1 led1 pbus64 tb3 jp1 22 pbus64 .
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